Please note: Plots (Histogram, Categorical Bar chart, Count and distribution, Pair plot, Box plot, Violin Plot, Joint plot, Scatter plot, Correlation plot) comparing the candidate genes with the other maize genes is generated using a randomly downsampled set of other maize genes. The size of the randomly downsampled genes is equal to the size of the user defined candidate genes.

Example Candidate gene list:

To demonstrate the potential use case of the user candidate gene analysis, we gathered a set of fifty stress genes that were differentially expressed between the control and salt stress samples and used them to identify unique characteristics common among salt stress genes .

Please go through the below explanation for details on table and plot interpretations

Table column interpretation:

1. Transmembrane helices(TMHMM)

Predicted transmembrane helices count in proteins. TMHMM, based on a hidden Markov model (HMM) approach was used to identify and model various regions of membrane protein: helix caps, middle of helix, regions close to the membrane, and globular domains. Further details about the programme can be found here:
https://people.binf.ku.dk/~krogh/publications/pdf/KroghEtal01.pdf

2. Coils

Predicted loops/coils count in proteins . Loops/coils as defined by DSSP algorithm. Residues are assigned as belonging to one of several secondary structure types. As per this defination we can consider residues as alpha -helix ('H'), 3_10-helix ('G') or beta-strand ('E') as ordered, and all other states ('T', 'S', 'B', 'I', ' ') as loops (also known as coils). Loops/coils are not necessarily disordered, however protein disorder is only found within loops. It follows that one can use loop assignments as a necessary but not sufficient requirement for disorder. For further details on the prediction tool,follow the following link:
http://dis.embl.de/

3. REM45

Predicted REM45 count in proteins. REM45 are missing coordinates in X-Ray structure as defined by REMARK-465 entries in PDB. Non assigned electron densities most often reflect intrinsic disorder, and have been used early on in disorder prediction. For further details on the prediction tool,follow the following link:
http://dis.embl.de/

4. Hotloops

Predicted Hotloops count in proteins. Hot loops constitute a subset of the ordered states, namely those loops with a high degree of mobility as determined from C-alpha temperature (B-)factors. It follows that highly dynamic loops should be considered protein disorder. For further details on the prediction tool,follow the following link:
http://dis.embl.de/

5. Signal Peptides

Predicted Signal Peptides in proteins. The SignalP 5.0 server predicts the presence of signal peptides and the location of their cleavage sites in proteins from Archaea, Gram-positive Bacteria, Gram-negative Bacteria and Eukarya. For further details on the prediction tool,follow the following link:
https://services.healthtech.dtu.dk/service.php?SignalP-5.0


6. Labels

The labels are the classes or the groups the genes are mapped into.The labels can act as both target variable or feature as per the need of the user for solving their specific problem

6.1 No Label

This selection is provided to enable users to view the properties of all genes without labeling them into different gene categories or annotations. This is to let users examine the features of multiple genes and identify common patterns among them. As it involves the inspection of all the genes therefore they work only for "Submit for analysis" button .

6.2 Classical Genes

Classical genes can be defined as the most well-studied genes mainly for their visible mutant phenotype (for example: liguleless3).

6.3 Pan-genome Genes

A gene in a given taxonomic group is either present in every individual (core), or absent in at least a single individual (dispensable).

6.4 Origin Genes

Gene duplication is an important evolutionary mechanism allowing new genetic material and thus opportunities to acquire new gene functions for an organism. There are different origins of duplications such as whole-genome duplications, tandems, etc.


Graph interpretations:

To the top right corner of the plots/graphs, there are options to download plot, zoom-out/zoom in, reset axes, autoscale, toggle spike lines, show closest data on hover, compare data on hover, box select, pan and lasso. Users can also select specific legends to view data only for the selected legends. Details on the interactive plot options are available here:
Interactive graph features

1. Marginal Plot

The Marginal plots are histrogram plot showing the frequency distribution of the selected gene features alonghwith higlighting the candidate genes. This plot will enable the user to easily identify where thier candidate gene lie among the other maize genes for the selected feature.

2. Histogram

The Histogram shows the frequency distribution of the selected protein structures such as Transmembrane helices, Coils,REM45 etc . The X-axis in the histogram represents the range of values present in the selected protein structure. The Y-axis represents the frequency of values. In addition to the graph,to increase the interpretability of the data, We have also included P-values,mean and standard deviations of the selected datasets.

3. Categorical marginal Plot

The Categorical marginal Plots are bar plots showing the frequency distribution of the different categories of the selected gene features alonghwith higlighting the candidate genes. This plot will enable the user to easily identify where thier candidate gene lie among the other maize genes for the selected feature.

4. Categorical Bar chart

The Categorical bar chart shows the frequency distribution of the different categories in the selected protein structures such as Signal Peptides. The X-axis in the Categorical bar chart represents the different categories in the selected protein structure. The Y-axis represents the frequency of the categories in the selected protein structure. In addition to the graph,to increase the interpretability of the data, We have also included P-values, mean and standard deviations of the selected datasets.

5. Count and distribution

The Count and distribution plot is a smoothed, continuous version of a histogram estimated from the data. The most common form of estimation is known as kernel density estimation.The x-axis is the value of the selected protein structures just like in a histogram and the y-axis in a distribution plot is the probability density function and not a probability. The difference is the probability density is the probability per unit on the x-axis. In general the y-axis on the distribution plot is a value only for relative comparisons between different categories like classical and other genes.

6. Pair plots

The pairs plot builds on two basic figures, the distributions and the scatter plot. The distributions on the diagonal allows us to see the distribution of a single selected codon indexes while the scatter plots on the upper and lower triangles show the relationship (or lack thereof) between two variables such as Coils and REM45.

7. Box plots

Boxplots are a measure of how well distributed the data in the selected protein structures are. It divides the data set into three quartiles. This graph represents the minimum, maximum, median, first quartile and third quartile in the selected protein structure. It is also useful in comparing the distribution of data across data sets by drawing boxplots for each of them such as the core, non-core, dispensable and private genes. Boxplots can be used to:

8. Violin plots

Violin plots are similar to box plots, except that they also show the probability density of the seleced protein structure at different values. These plots include a marker for the median of the data and a box indicating the interquartile range, as in the standard box plots. Overlaid on this box plot is a kernel density estimation. Like box plots, violin plots are used to represent comparison of a variable distribution such as Transmembrane helices, Coils,REM45 (or sample distribution) across different "categories" (Classical/Other or Core/Non-core) .
A violin plot is more informative than a plain box plot. In fact while a box plot only shows summary statistics such as mean/median and interquartile ranges, the violin plot shows the full distribution of the selected codon index.

9. Joint plots

A Jointplot comprises three plots. Out of the three, one plot displays a bivariate graph(scatter plot) which shows how the one variable( such as Coils) varies with the another variable(such as REM45). Another plot is placed horizontally at the top of the bivariate graph and it shows the distribution in the form of marks along an axis for each one of the selected protein structures (for example rug plot of Coils and REM45 ). The third plot is placed on the right margin of the bivariate graph with the orientation set to vertical and it shows the distribution of again the two selected protein structures .
It is very helpful to have univariate and bivariate plots together in one figure. This is because the univariate analysis focuses on one variable, it describes, summarizes and shows any patterns in your data and the bivariate analysis explores the relationship between two variables and also describes the strength of their relationship.

10. Scatter plots

A Scatter plot is a great way of exploring relationships or patterns in data. But adding a regression line can make those patterns stand out . Therefore Scatter plot with simple linear regression for the selected protein structures, explains the strength of the relationship between the two variables such as Coils or REM45 in your scatter-plot using R2, the squared correlation coefficient.It is always between 0 and 1. Higher R2 indicates stronger relationship between the selected protein structures .

11. Correlation plots

Correlation heatmap is graphical representation of correlation matrix representing correlation between different selected protein structures. Correlation ranges from -1 to +1. Values closer to zero means there is no linear trend between the two variables. The closer to 1 the correlation is the more positively correlated they are; that is as one increases so does the other and the closer to 1 the stronger this relationship is. Correlation plots also alerts us to potential multicollinearity problems.

12.Dendrogram plots

A dendrogram is a type of tree diagram showing hierarchical clustering — relationships between similar sets of genes based on the selected protein structures. They are frequently used in biology to show clustering between genes or samples, but they can represent any type of grouped data.
The branches in the dendrogram are arranged according to how similar (or dissimilar) they are. Branches that are close to the same height are similar to each other; branches with different heights are dissimilar — the greater the difference in height, the more dissimilarity. Also the different clusters of genes based on the selected protein structures are marked by different colors.

13. Hierarchical Scatterplot

The Hierarchical Scatter plot is a type of pair plot that can be used to visualize the relationship of different pairs of protein structures on the clusters of genes formed during dendrogram.Therefore to create Hierarchical scatterplot we need to input the number of clusters in the choose cluster input box. For the Hierarchical Scatter plot, we need to specify the number of clusters we want to view. Moreover, this number of clusters corresponds to the number of clusters formed in the Dendrogram plot, so we perform this analysis only after studying the Dendrogram plot. The Hierarchical Scatter plot is dynamically sized for the number of clusters, so every user can analyze the dataset based on how many clusters they believe the selected data is forming .

14. Hierarchical Heatmap

Hierarchical clustering heatmap is an intuitive way to visualize information from complex data. It’s also called a false colored image, where data values are transformed to color scale. Heat maps allow us to simultaneously visualize clusters of samples(selected protein structures) in the column and features (genes) in the rows.
First hierarchical clustering is done of both the rows and the columns of the data matrix. The columns/rows of the data matrix are re-ordered according to the hierarchical clustering result, putting similar observations close to each other. The blocks of ‘high’ and ‘low’ values are adjacent in the data matrix. Finally, a color scheme is applied for the visualization and the data matrix is displayed. Visualizing the data matrix in this way can help to find the variables(selected protein structures) that appear to be characteristic for each sample cluster.

15. Heatmap

A heatmap is a plot of rectangular data as a color-encoded matrix.This is a great way to visualize data, because it can show the relation between variables (selected protein structure's) including genes. The heatmap here shows the relation between the first 100 genes and the selected protein structure's values to reduce time and complexity .

PCA

PCA reduces the high-dimensional interrelated data to low-dimension by linearly transforming the old variable into a new set of uncorrelated variables called principal component (PC) while retaining the most possible variation.
The first component has the largest variance followed by the second component and so on. The first few components retain most of the variation, which is easy to visualize and summarize the features of original high-dimensional datasets in low-dimensional space. PCA helps to assess which original samples are similar and different from each other.
In our case when the selected number of protein structure's are higher than 2 then, it is arduous to visualize them at the same time to interpret the genes.PCA transforms them into a new set of variables (PCs) with top PCs having the highest variation. PCs are ordered which means that the first few PCs (generally first 3 PCs but can be more) contribute most of the variance present in the original high-dimensional dataset. These top first 2 or 3 PCs can be plotted easily and summarize the features of all original variables (selected protien structures).

16. PCA 2D variables cluster

A PCA 2D variables cluster plot shows how strongly each characteristic or variable influences a principal component. In the plot we can see these vectors(variables) are pinned at the origin of PCs (PC1 = 0 and PC2 = 0). Their project values on each PC show how much weight they have on that PC.

17. PCA 2D observation cluster

A PCA 2D observation cluster plot showing clusters of samples/observations based on their similarity.PCA does not discard any samples or observations. Instead, it reduces the overwhelming number of dimensions by constructing principal components (PCs). PCs describe variation and account for the varied influences of the original characteristics. Such influences, or loadings, can be traced back from the PCA plot to find out what produces the differences among clusters.
Observations further out are either outliers or naturally extreme observations. Plot observations are annotated with shapes and colors to highlight gene models and gene types for example (Classical/Others). In this way, we can easily hover over any data point, especially outliers, to find gene models with extreme observation values across various sample types. (selected protein structures)

18. PCA 2D biplot cluster

PCA biplot = PCA observations plot + variable plot.
we can probably notice that a PCA biplot simply merges an usual observations PCA plot with a plot of variables. The arrangement is like this:
*Bottom axis: PC1 score.
* Left axis: PC2 score.
In other words, the left and bottom axes are of the PCA observations plot — use them to read PCA scores of the observations (dots).

Another nice thing about these plots: the angles between the vectors/variables tell us how characteristics or variables correlate with one another.
* When two vectors are close, forming a small angle, the two variables they represent are positively correlated.
*If they meet each other at 90°, they are not likely to be correlated.
*When they diverge and form a large angle (close to 180°), they are negatively correlated.

19. PCA 3D variables cluster

This is similar to the PCA 2D variables cluster plot but now instead of two principal components, we can have three PC'S that contribute to most of the variance present in the original high-dimensional dataset. These top 3 PCs can be plotted easily in 3 dimensional space and summarize the features of all original variables (selected codon index's) .

20. PCA 3D observation cluster

This is similar to the PCA 2D observation cluster but now instead of two principal components, we can have three PC'S that contribute to most of the variance present in the original high-dimensional dataset. These top 3 PCs can be plotted easily in 3 dimensional space and summarize the variance in the observations .
A PCA 3D observation cluster plot displays how much variation each principal component captures from the data. If the first three PCs are sufficient to describe the essence of the data.